الكلية: الصيدلة والعلوم الطبية
الجهة البحثية: جامعة البترا
عنوان البحث المنشور:
Nanobody-based immunosensor for the detection of H. pylori in saliva
سنة النشر: 2024
ملخص البحث المنشور:
Helicobacter pylori (H. pylori) infection is highly prevalent worldwide, affecting more than 43% of world popu lation. The infection can be transmitted through different routes, like oral-oral, fecal-oral, and gastric-oral. Electrochemical sensors play a crucial role in the early detection of various substances, including bio molecules. In this study, the development of nanobody (Nb)-based immunosensor for the detection of H. pylori antigens in saliva samples was investigated. The D2_Nb was isolated and characterized using Western blot and ELISA and employed in the fabrication of the immunosensor. The sensor was prepared using gold screen-printed electrodes, with the immobilization of Nb achieved through chemical linkage using cysteamine-glutaraldehyde. The surface of the electrode was characterized using EIS, FTIR and SEM. Initially, the Nb-based immunosensor’s performance was evaluated through cyclic voltammetry (CV), differential pulse voltammetry (DPV), and square wave voltammetry (SWV). The sensor exhibited excellent linearity with an R2 value of 0.96. However, further assessment with the DPV technique revealed both a low limit of detection (5.9 ng/mL, <1 cfu/mL) and high selectivity when exposed to a mixture of similar antigens. Moreover, the immunosensor demonstrated robust recovery rates (96.2%–103.4%) when spiked into artificial saliva and maintained its functionality when stored at room temperature for 24 days.
رابط البحث المنشور:
https://www.sciencedirect.com/science/article/abs/pii/S0956566324004287